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  Indian J Med Microbiol
 

Figure 2: Radiochromatograms showing the behavior of labeled gold, hydrolyzed (colloidal) gold and free (control) gold in different solvent mediums: The thin layer chromatography spots when developed in solvent mediums such as normal saline and acetone, then the radiolabeled ligand, as well as the hydrolyzed (colloidal) radiometal, remain at the point of spotting, while the free radiometal moves with the solvent front. However, using the system of “triple mixture solvent” (ethanol:water:ammonia – 2:5:1) the hydrolyzed radiometal stays at the origin and the radiolabeled compound like the free radiometal moves with the solvent front

Figure 2: Radiochromatograms showing the behavior of labeled gold, hydrolyzed (colloidal) gold and free (control) gold in different solvent mediums: The thin layer chromatography spots when developed in solvent mediums such as normal saline and acetone, then the radiolabeled ligand, as well as the hydrolyzed (colloidal) radiometal, remain at the point of spotting, while the free radiometal moves with the solvent front. However, using the system of “triple mixture solvent” (ethanol:water:ammonia – 2:5:1) the hydrolyzed radiometal stays at the origin and the radiolabeled compound like the free radiometal moves with the solvent front