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  Indian J Med Microbiol
 

Figure 1: Chromatographic spots imaged with Siemens Orbitor Gamma Camera ZLC-7500 showing comparative labeling for both vial (1) and vial (2) reaction mixtures. The resultant reaction mixtures of the two vials (1) and (2) were spotted on the instant thin layer chromatography paper sheet and were subjected to thin layer chromatography in three different systems – normal saline, acetone, and triple solvent mixture (normal saline-as a solvent medium shown in a figure). For comparison, free (control) radio gold was also run with these. All the strips were imaged with our Siemens Orbitor Gamma Camera ZLC-7500 and the pictures acquired on the computer. The amount of radiolabeling was estimated by both visual inspections of these images as well as by in vitro counting of the quarters of the chromatograph strips. It was observed that in all the three systems free pertechnetate moved with the solvent front, with nothing or very little remaining at the origin. Labeling was observed with both the reaction mixtures, with a considerable amount remaining at the origin. However, the amount that remains at the origin is significantly higher for the reaction mixture of vial (1) (gold nanoparticle labeled immunoglobulin) in comparison to that for a vial (2) (gold ordinary particulate labeled immunoglobulin), representing better labeling of an antibody with radioactive nanogold. Labeling efficiency was reported to be optimized, up to >85%–90% using nanogold particles of precise size and shape

Figure 1: Chromatographic spots imaged with Siemens Orbitor Gamma Camera ZLC-7500 showing comparative labeling for both vial (1) and vial (2) reaction mixtures. The resultant reaction mixtures of the two vials (1) and (2) were spotted on the instant thin layer chromatography paper sheet and were subjected to thin layer chromatography in three different systems – normal saline, acetone, and triple solvent mixture (normal saline-as a solvent medium shown in a figure). For comparison, free (control) radio gold was also run with these. All the strips were imaged with our Siemens Orbitor Gamma Camera ZLC-7500 and the pictures acquired on the computer. The amount of radiolabeling was estimated by both visual inspections of these images as well as by<b> in vitro </b> counting of the quarters of the chromatograph strips. It was observed that in all the three systems free pertechnetate moved with the solvent front, with nothing or very little remaining at the origin. Labeling was observed with both the reaction mixtures, with a considerable amount remaining at the origin. However, the amount that remains at the origin is significantly higher for the reaction mixture of vial (1) (gold nanoparticle labeled immunoglobulin) in comparison to that for a vial (2) (gold ordinary particulate labeled immunoglobulin), representing better labeling of an antibody with radioactive nanogold. Labeling efficiency was reported to be optimized, up to >85%–90% using nanogold particles of precise size and shape