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Year : 2014  |  Volume : 29  |  Issue : 4  |  Page : 276-277  

Detection of homing-in of stem cells labeled with technetium-99m hexamethylpropyleneamine oxime in infarcted myocardium after intracoronary injection


1 Department of Nuclear Medicine, All India Institute of Medical Sciences, New Delhi, India
2 Department of Cardiology, All India Institute of Medical Sciences, New Delhi, India
3 Stem Cell Facility, All India Institute of Medical Sciences, New Delhi, India

Date of Web Publication11-Oct-2014

Correspondence Address:
Sujata Mohanty
Stem Cell Facility, Ist Floor, ORBO Complex, All India Institute of Medical Sciences, New Delhi - 110 029
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0972-3919.142647

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   Abstract 

Bone marrow stem cells having myogenic potential are promising candidates for various cell-based therapies for myocardial disease. We present here images showing homing of technetium-99m (Tc-99m) hexamethylpropyleneamine oxime (HMPAO) labeled stem cells in the infarcted myocardium from a pilot study conducted to radio-label part of the stem cells in patients enrolled in a stem cell clinical trial for recent myocardial infarction.

Keywords: Bone marrow mononuclear cells, myocardium, stem cell, technetium-99m hexamethylpropyleneamine oxime


How to cite this article:
Patel CD, Agarwal S, Seth S, Mohanty S, Aggarwal H, Gupta N. Detection of homing-in of stem cells labeled with technetium-99m hexamethylpropyleneamine oxime in infarcted myocardium after intracoronary injection. Indian J Nucl Med 2014;29:276-7

How to cite this URL:
Patel CD, Agarwal S, Seth S, Mohanty S, Aggarwal H, Gupta N. Detection of homing-in of stem cells labeled with technetium-99m hexamethylpropyleneamine oxime in infarcted myocardium after intracoronary injection. Indian J Nucl Med [serial online] 2014 [cited 2019 Dec 12];29:276-7. Available from: http://www.ijnm.in/text.asp?2014/29/4/276/142647

We present here a case of 42-year-old male with a history of myocardial infarction (MI) and angioplasty 1 month back. Resting Tc-99m methoxyisobutylisonitrile (MIBI) myocardial perfusion study was done to confirm and locate the site of MI. On separate day, the patient underwent a bone marrow (BM) aspirate collection. BM derived mononuclear cells (MNCs) were isolated by Ficoll density gradient separation method. [1],[2] The isolated MNCs were evaluated for viability by Trypan Blue dye exclusion test, surface marker profile for CD 34 by flow cytometry and MNC morphology by Giemsa staining.

Bone marrow-MNCs were incubated under sterile conditions for 45 min at room temperature with 10 mCi of Tc-99m HMPAO in 1 ml normal saline (NS) followed by centrifugation and decanting. Excess of unbound activity was removed by two washings of resuspended pellet in 1 ml NS followed by centrifugation. This pellet was finally diluted in NS. Freshly isolated MNCs showed 65% labeling efficiency and intact viability. A dose of 2.5 mCi of radio labeled BM-MNCs was withdrawn and intracoronary injection into the left coronary arteries was performed in cardiac catheterization laboratory. The images were acquired within 1 h postinjection on Dual head Gamma camera system (Infinia Hawkeye, GE Medical system, USA) and homing-in of radiolabel stem cells was observed [Figure 1].
Figure 1: (a) The short axis images of technetium-99m (Tc-99m)-MIBI myocardial perfusion single-photon emission computed tomography (SPECT) performed at rest reveal perfusion defect in anterior wall and apex; consistent with scarred myocardium (upper row). (b) Planar image showing the biodistribution of Tc-99m- MIBI. (c) The corresponding short axis images of Tc-99m-HMPAO labeled stem cells SPECT study show localization of labeled stem cells in the region of infarct (lower row). (d) Planar image showing the biodistribution of Tc-99m-HMPAO labeled stem cells in liver, spleen and infarcted myocardium

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Bone marrow stem cells having myogenic potential are promising candidates for various cell-based therapies for myocardial disease. Present images show homing-in of Tc-99m HMPAO labeled stem cells in the infarcted myocardium. Previous studies have reported the Technetium labeled stem cells in animal studies [3],[4],[5] and also its feasibility in humans. [6],[7],[8] A pilot study was conducted to radio-label part of the stem cells in patients enrolled in a stem cell clinical trial for recent MI.

 
   References Top

1.
Hibino N, Nalbandian A, Devine L, Martinez RS, McGillicuddy E, Yi T, et al. Comparison of human bone marrow mononuclear cell isolation methods for creating tissue-engineered vascular grafts: Novel filter system versus traditional density centrifugation method. Tissue Eng Part C Methods 2011;17:993-8.  Back to cited text no. 1
    
2.
Goodchild TT, Robinson KA, Pang W, Tondato F, Cui J, Arrington J, et al. Bone marrow-derived B cells preserve ventricular function after acute myocardial infarction. JACC Cardiovasc Interv 2009;2:1005-16.  Back to cited text no. 2
    
3.
Barbash IM, Chouraqui P, Baron J, Feinberg MS, Etzion S, Tessone A, et al. Systemic delivery of bone marrow-derived mesenchymal stem cells to the infarcted myocardium: Feasibility, cell migration, and body distribution. Circulation 2003;108:863-8.  Back to cited text no. 3
    
4.
Becerra P, Valdés Vázquez MA, Dudhia J, Fiske-Jackson AR, Neves F, Hartman NG, et al. Distribution of injected technetium (99m)-labeled mesenchymal stem cells in horses with naturally occurring tendinopathy. J Orthop Res 2013;31:1096-102.  Back to cited text no. 4
    
5.
Park BN, Shim W, Lee G, Bang OY, An YS, Yoon JK, et al. Early distribution of intravenously injected mesenchymal stem cells in rats with acute brain trauma evaluated by (99m) Tc-HMPAO labeling. Nucl Med Biol 2011;38:1175-82.  Back to cited text no. 5
    
6.
Kollaros N, Theodorakos A, Manginas A, Kitziri E, Katsikis A, Cokkinos D, et al. Bone marrow stem cell adherence into old anterior myocardial infarction: A scintigraphic study using Tl-201 and Tc-99m-HMPAO. Ann Nucl Med 2012;26:228-33.  Back to cited text no. 6
    
7.
Musialek P, Tekieli L, Kostkiewicz M, Majka M, Szot W, Walter Z, et al. Randomized transcoronary delivery of CD34(+) cells with perfusion versus stop-flow method in patients with recent myocardial infarction: Early cardiac retention of (99m) Tc-labeled cells activity. J Nucl Cardiol 2011;18:104-16.  Back to cited text no. 7
    
8.
McColgan P, Sharma P, Bentley P. Stem cell tracking in human trials: A meta-regression. Stem Cell Rev 2011;7:1031-40.  Back to cited text no. 8
    


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